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cyflow space facs machine  (Partec)
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Partec cyflow space facs machine
ChR2 expression and characterization in hiPSC derived cardiomyocytes. ( A ) Schema of the adeno-associated virus (AAV) construct for expression of ChR2 and mCherry under the control of the chicken β-actin (CAG) promoter (top) and illustration of ChR2, a light-induced non-selective cation channel (bottom). ( B ) mCherry signals (red) indicating ChR2 expression in α-actinin positive (green) cardiomyocytes (nuclear staining in blue, scale bar: 100 µm). ( C ) Representative example of <t>FACS</t> analysis of mCherry positive cardiomyocytes after AAV transduction. ( D ) Representative current trace during illumination (5 mW/mm 2 ) at a holding potential of −40 mV. ( E ) Quantification of light-induced peak- and steady state photocurrents (n = 19 from 1 batch). ( F ) Overlay of APs induced by electrical (400 pA current injection, 2 ms, black) and optical stimulation (1.4 mW/mm 2 , 1 ms, blue). ( G ) Action potential duration at 20%, 30%, 50%, 70% and 90% of repolarization (APD) during electrical and optical pacing (APD20: p = 0.1995; APD30: p = 0.4027; APD50: p = 0.3064; APD70: p = 0.2647; APD90: p = 0.1893, n = 13 from 1 batch, two-tailed, paired student’s t -test).
Cyflow Space Facs Machine, supplied by Partec, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cyflow space facs machine - by Bioz Stars, 2026-04
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ChR2 expression and characterization in hiPSC derived cardiomyocytes. ( A ) Schema of the adeno-associated virus (AAV) construct for expression of ChR2 and mCherry under the control of the chicken β-actin (CAG) promoter (top) and illustration of ChR2, a light-induced non-selective cation channel (bottom). ( B ) mCherry signals (red) indicating ChR2 expression in α-actinin positive (green) cardiomyocytes (nuclear staining in blue, scale bar: 100 µm). ( C ) Representative example of FACS analysis of mCherry positive cardiomyocytes after AAV transduction. ( D ) Representative current trace during illumination (5 mW/mm 2 ) at a holding potential of −40 mV. ( E ) Quantification of light-induced peak- and steady state photocurrents (n = 19 from 1 batch). ( F ) Overlay of APs induced by electrical (400 pA current injection, 2 ms, black) and optical stimulation (1.4 mW/mm 2 , 1 ms, blue). ( G ) Action potential duration at 20%, 30%, 50%, 70% and 90% of repolarization (APD) during electrical and optical pacing (APD20: p = 0.1995; APD30: p = 0.4027; APD50: p = 0.3064; APD70: p = 0.2647; APD90: p = 0.1893, n = 13 from 1 batch, two-tailed, paired student’s t -test).

Journal: Scientific Reports

Article Title: Frequency-dependent drug screening using optogenetic stimulation of human iPSC-derived cardiomyocytes

doi: 10.1038/s41598-017-09760-7

Figure Lengend Snippet: ChR2 expression and characterization in hiPSC derived cardiomyocytes. ( A ) Schema of the adeno-associated virus (AAV) construct for expression of ChR2 and mCherry under the control of the chicken β-actin (CAG) promoter (top) and illustration of ChR2, a light-induced non-selective cation channel (bottom). ( B ) mCherry signals (red) indicating ChR2 expression in α-actinin positive (green) cardiomyocytes (nuclear staining in blue, scale bar: 100 µm). ( C ) Representative example of FACS analysis of mCherry positive cardiomyocytes after AAV transduction. ( D ) Representative current trace during illumination (5 mW/mm 2 ) at a holding potential of −40 mV. ( E ) Quantification of light-induced peak- and steady state photocurrents (n = 19 from 1 batch). ( F ) Overlay of APs induced by electrical (400 pA current injection, 2 ms, black) and optical stimulation (1.4 mW/mm 2 , 1 ms, blue). ( G ) Action potential duration at 20%, 30%, 50%, 70% and 90% of repolarization (APD) during electrical and optical pacing (APD20: p = 0.1995; APD30: p = 0.4027; APD50: p = 0.3064; APD70: p = 0.2647; APD90: p = 0.1893, n = 13 from 1 batch, two-tailed, paired student’s t -test).

Article Snippet: Percentage of mCherry expressing Cor4U cardiomyocytes was analysed with a CyFlow Space FACS machine (Partec GmbH, Münster, Germany, 561 nm laser excitation, 590 ± 50 nm emission).

Techniques: Expressing, Derivative Assay, Virus, Construct, Control, Staining, Transduction, Injection, Two Tailed Test